Activation of caspase-3 in gastric adenocarcinoma AGS cells by Xylopia aethiopica (Dunal) A. Rich. fruit and characterization of its phenolic fingerprint by HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Ribeiro, V (Autor) Outra; Ferreres, F (Autor) Outra; Macedo, T (Autor) Outra; Gil Izquierdo, A (Autor) Outra; Oliveira, AP (Autor) Outra; Gomes, NGM (Autor) Outra; Araujo, L (Autor) Outra; David M Pereira (Autor) FFUP; Paula B Andrade (Autor) FFUP; Patricia Valentao (Autor) FFUP

Revista

Título: Food Research International

Vol. 141

ISSN: 0963-9969

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00T-BK1

DOI: 10.1016/j.foodres.2021.110121

Abstract (EN): While the fruits of Xylopia aethiopica (Dunal) A. Rich. are important in African countries as a local trade product, their composition remains scarcely investigated. Phenolic fingerprint is herein delivered through HPLC-DAD-ESI (Ion Trap)-MSn and UPLC-ESI-QTOF-MS2 analysis, six cinnamoylquinic acid derivatives and twenty-four flavonoid glycosides being determined, chrysoeriol-7-O-glycosides being the main constituents. A cytotoxicity screening of twenty-eight hydroethanol extracts, obtained from a collection of Guinea-Bissauan plants, against A549 and AGS carcinoma cells, revealed the selective and potent effect towards AGS cells (IC50 = 151 x 10(-3) g L-1), upon exposure to the extract from X. aethiopica fruits. Additional experiments demonstrated insignificant effect on LDH release at 151 x 10(-3) g L-1, morphological analysis further suggesting induction of apoptosis. Pro-apoptotic effects were confirmed, as the extract enabled the activation of the effector caspase-3, broadening the knowledge on the anticancer mechanisms elicited by the fruits of X. aethiopica. Phenolic constituents might contribute to the cytotoxic effects, particularly via caspase-3 activation. Considering that X. aethiopica fruit is very often referred as an anticancer ingredient in Africa, but mainly the potent cytotoxicity herein recorded, our results call for additional research aiming to identify non-phenolic constituents contributing to the effects and also to further detail the anticancer mechanisms.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10