Optimisation, by response surface methodology, of degree of hydrolysis and antioxidant and ACE-inhibitory activities of whey protein hydrolysates obtained with cardoon extract

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Tavares, TG (Autor) Outra; Contreras, MM (Autor) Outra; Amorim, M (Autor) Outra; Martin Alvarez, PJ (Autor) Outra; Pintado, ME (Autor) Outra; Recio, I (Autor) Outra; F. Xavier Malcata (Autor) Outra

Revista

Título: International Dairy Journal

Vol. 21 Nº 3

Páginas: 926-933

ISSN: 0958-6946

Editora: Elsevier

Indexação

ISI Web of Knowledge - 44 Citações

Scopus - 55 Citações

Outras Informações

ID Authenticus: P-002-JFB

DOI: 10.1016/j.idairyj.2011.05.013

Abstract (EN): The hydrolysis of bovine whey protein concentrate (WPC), alpha-lactalbumin (alpha-La) and caseinomacropeptide (CMP), by aqueous extracts of Cynara cardunculus, was optimized using response surface methodology. Degree of hydrolysis (DH), angiotensin-converting enzyme (ACE)-inhibitory activity and antioxidant activity were used as objective functions, and hydrolysis time and enzyme/substrate ratio as manipulated parameters. The model was statistically appropriate to describe ACE-inhibitory activity of hydrolysates from WPC and alpha-La, but not from CMP. Maximum DH was 18% and 9%, for WPC and alpha-La, respectively. 50% ACE-inhibition was produced by 105.4 (total fraction) and 25.6 mu g mL(-1) (<3 kDa fraction) for WPC, and 47.6 (total fraction) and 22.5 mu g mL(-1) (<3 kDa fraction) for alpha-La. Major peptides of fractions exhibiting ACE-inhibition were sequenced. The antioxidant activities of WPC and alpha-La were 0.96 +/- 0.08 and 1.12 +/- 0.13 mmol trolox equivalent per mg hydrolysed protein, respectively. (c) 2011 Published by Elsevier Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8