Characterization of a panel of monoclonal antibodies using GalNAc glycosylated peptides and recombinant MUC1

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

Celso Reis (Autor) Outra; Hassan, H (Autor) Outra; Bennett, EP (Autor) Outra; Clausen, H (Autor) Outra

Revista

Título: Tumor Biology

Vol. 19

Páginas: 127-133

ISSN: 1010-4283

Editora: SAGE

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 8 Citações

Outras Informações

ID Authenticus: P-001-9TJ

DOI: 10.1159/000056514

Abstract (EN): A panel of 56 murine monoclonal antibodies submitted to the ISOBM TD-4 (MUC1) Workshop was analysed for reactivity against nonglycosylated and in vitro Ga1NAc glycosylated peptides. Twenty-six antibodies reacted with nonglycosylated MUC1 peptides containing 3-5 tandem repeats, The reactivities of most of the antibodies were not affected by Ga1NAc glycosylation of the peptides, Antibody #147 (decoded as BCP9) reactivity was inhibited when 3 mol of Ga1NAc per repeat were incorporated in the peptide (at sites T in GVTSA and ST in GSTAP), whereas the reactivity with Ga1NAc glycosylated peptides with 2 mol of Ga1NAc per repeat (sites T in GVTSA and T in GSTAP) was unaffected, This is in agreement with the epitope defined as GSTAP.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7