Characterization of a panel of monoclonal antibodies using GalNAc glycosylated peptides and recombinant MUC1

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Celso Reis (Author) Other; Hassan, H (Author) Other; Bennett, EP (Author) Other; Clausen, H (Author) Other

Journal

Title: Tumor Biology

Vol. 19

Pages: 127-133

ISSN: 1010-4283

Publisher: SAGE

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-001-9TJ

DOI: 10.1159/000056514

Abstract (EN): A panel of 56 murine monoclonal antibodies submitted to the ISOBM TD-4 (MUC1) Workshop was analysed for reactivity against nonglycosylated and in vitro Ga1NAc glycosylated peptides. Twenty-six antibodies reacted with nonglycosylated MUC1 peptides containing 3-5 tandem repeats, The reactivities of most of the antibodies were not affected by Ga1NAc glycosylation of the peptides, Antibody #147 (decoded as BCP9) reactivity was inhibited when 3 mol of Ga1NAc per repeat were incorporated in the peptide (at sites T in GVTSA and ST in GSTAP), whereas the reactivity with Ga1NAc glycosylated peptides with 2 mol of Ga1NAc per repeat (sites T in GVTSA and T in GSTAP) was unaffected, This is in agreement with the epitope defined as GSTAP.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7