A Role for MicroRNA-155 Expression in Microenvironment Associated to HPV-Induced Carcinogenesis in K14-HPV16 Transgenic Mice

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Isabel Paiva (Autor) Outra; Rui M. Gil da Costa (Autor) FEUP; Joana Ribeiro (Autor) Outra; Hugo Sousa (Autor) ICBAS; Margarida M. S. M. Bastos (Autor) FEUP; Ana Faustino Carlos Rocha (Autor) Outra; Paula A. Oliveira (Autor) Outra; Rui Medeiros (Autor) ICBAS

Revista

Título: PLoS ONE

Vol. 10

Páginas: 1-14

ISSN: 1932-6203

Editora: Public Library of Science

Indexação

ISI Web of Knowledge - 33 Citações

Scopus - 36 Citações

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00A-6XZ

DOI: 10.1371/journal.pone.0116868

Abstract (EN): Human Papillomavirus cause a number of diseases most notably cervical cancer. K14-HPV16 transgenic mice expressing the HPV16 early genes in squamous epithelial cells provide a suitable experimental model for studying these diseases. MicroRNAs are small non-coding RNAs that play an important role in regulating gene expression and have been suggested to play an important role in cancer development. The role of miR-155 in cancer remains controversial and there is limited evidence linking this miRNA to HPV-associated diseases. We hypothesized that miR-155 expression modulates each tissue's susceptibility to develop HPV-associated carcinogenesis. In this study, we analyzed miR-155 expression in ear and chest skin samples from 22-26 weeks old, female K14-HPV16 transgenic (HPV16+/-) and wild-type (HPV-/-) mice. Among wild-type mice the expression of miR-155 was lower in ear skin compared with chest skin (p = 0.028). In transgenic animals, in situ carcinoma was present in all ear samples whereas chest tissues only showed epidermal hyperplasia. Furthermore, in hyperplastic chest skin samples, miR-155 expression was lower than in normal chest skin (p = 0,026). These results suggest that miR-155 expression may modulate the microenvironmental susceptibility to cancer development and that high miR155 levels may be protective against the carcinogenesis induced by HPV16.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 14