NO Fluorescence Quantification by Chitosan CdSe Quantum Dots Nanocomposites

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Eliana F C Simoes (Autor) Outra; Joao M M Leitao (Autor) Outra; Joaquim C G E Esteves da Silva (Autor) FCUP

Revista

Título: Journal of Fluorescence

Vol. 24

Páginas: 639-648

ISSN: 1053-0509

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 9 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-008-XFN

DOI: 10.1007/s10895-013-1337-0

Abstract (EN): The quantification of nitric oxide (NO) based on the quenching of the fluorescence of a nanocomposites sensor constituted by cadmium/selenium quantum dots (CdSe) stabilized by chitosan (CS) and mercaptosuccinic acid (MSA) is assessed. The optimization of the response of the CS-CdSe-MSA nanocomposites to NO was done by multivariate response surface experimental design methodologies. The highest fluorescence quenching was obtained at pH 5.5 and at room temperature. The NO quantification capability of CS-CdSe-MSA was evaluated using standard solutions and a NO donor reagent. A large linear working range from 5 to 200 mu M and a limit of detection of 1.86 mu M were obtained. Better quantification results were obtained using the NO donor reagent. Besides NO, the response of the fluorescence of CS-CdSe-MSA to the main reactive oxygen and nitrogen species and similar NO compounds was also assessed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10