Identification of luciferyl adenylate and luciferyl coenzyme A synthesized by firefly luciferase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

fraga, h (Autor) Outra; da silva, jcge (Autor) FCUP; fontes, r (Autor) FMUP

Revista

Título: ChemBioChem

Vol. 5

Páginas: 110-115

ISSN: 1439-4227

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 37 Citações

Scopus - 38 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-BZQ

DOI: 10.1002/cbic.200300735

Abstract (EN): The firefly luciferase reaction intermediate luciferyl adenylate was detected by RP-HPLC analysis when the luciferase reaction was performed under a nitrogen atmosphere. Although this compound is always specified as an intermediate in the light-production reaction, this is the first report of its identification by HPLC in a luciferase assay medium. Under a low-oxygen atmosphere, luciferase can catalyze the synthesis of luciferyl coenzyme A from luciferin, ATP, and coenzyme A, but in air dehydroluciferyl coenzyme A was produced. The luciferase-catalyzed synthesis of these coenzyme A derivatives may be a consequence of the postulated recent evolutionary origin of firefly luciferases from an ancestral acyl-coenzyme A synthetase.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6