Direct kinetic assay of interactions between small peptides and immobilized antibodies using a surface plasmon resonance biosensor

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Gomes, P (Autor) FCUP; Andreu, D (Autor) Outra

Revista

Título: Journal of Immunological Methods

Vol. 259

Páginas: 217-230

ISSN: 0022-1759

Editora: Elsevier

Indexação

ISI Web of Knowledge - 36 Citações

Scopus - 40 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-000-RF0

DOI: 10.1016/s0022-1759(01)00503-8

Abstract (EN): A surface plasmon resonance (SPR) protocol is described for the direct kinetic analysis of small antigenic peptides interacting with immobilized monoclonal antibodies (mAb). High peptide concentrations (up to 2.5 muM) and medium mAb surface densities (about 1.5 ng/mm(2)) are needed to ensure measurable binding levels, and fast buffer flow rates (60 mul/min) are required to minimize diffusion-controlled kinetics. Good reproducibility levels in the kinetic constants are obtained under these analysis conditions (standard deviations below 10% of the mean values). Application of this protocol to determine the antigenic ranking of viral peptides shows an excellent agreement between SPR and previous competition enzyme-link-ed immunosorbent assays (ELISA) on the same peptide/antibody systems.

Idioma: Inglês

Tipo (Avaliação Docente): Científica