Identification and characterization of a polyomavirus in the thornback skate (Raja clavata)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Abrantes, J (Autor) FCUP; Varsani, A (Autor) Outra; Pereira, P (Autor) Outra; Maia, C (Autor) Outra; Farias, I (Autor) Outra; Verissimo, A (Autor) FCUP; Neves, F (Autor) Outra

Revista

Título: Virology Journal

Vol. 10

ISSN: 1743-422X

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 5 Citações

Outras Informações

ID Authenticus: P-00Z-03F

DOI: 10.1186/s12985-023-02149-1

Abstract (EN): Members of the family Polyomaviridae have a circular double-stranded DNA genome that have been identified in various hosts ranging from mammals to arachnids. Here we report the identification and analysis of a complete genome sequence of a novel polyomavirus, Raja clavata polyomavirus (RcPyV1), from a cartilaginous fish, the thornback skate (Raja clavata). The genome sequence was determined using a metagenomics approach with an aim to provide baseline viral data in cartilaginous fish in different ecosystems. The RcPyV1 genome (4,195 nucleotides) had typical organization of polyomavirus, including early antigens (small T; Large T) encoded on one strand and late viral proteins (VP1; VP2) on the complementary strand. Maximum-likelihood phylogenetic analysis of the large T-antigen revealed that RcPyV1 clusters with a polyomavirus obtained from another cartilaginous fish, the guitarfish polyomavirus 1 (GfPyV1). These two share similar to 56% pairwise identity in LT and VP1 protein sequences. These analyses support the hypothesis that cartilaginous fishes have a specific lineage of polyomaviruses.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7