The effect of glucose concentration and shaking conditions on Escherichia coli biofilm formation in microtiter plates

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Joana M. R.Moreira (Autor) FEUP; Luciana C. Gomes (Autor) FEUP; José D. P. Araújo (Autor) FEUP; João M. Miranda (Autor) FEUP; Manuel Simões (Autor) FEUP; Luís F. Melo (Autor) FEUP; Filipe J. Mergulhão (Autor) FEUP

Revista

Título: Chemical Engineering Science

Vol. 94 Nº MAY 3 2013

Páginas: 192-199

ISSN: 0009-2509

Editora: Elsevier

Indexação

ISI Web of Knowledge - 29 Citações

ISI Web of Science

Scopus - 36 Citações

INSPEC

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia química

Projetos Associados

SYNBIOBACTHER - Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética

Filmstart - Estudo das condições iniciais que controlam a formação de biofilmes

Outras Informações

ID Authenticus: P-005-0T9

DOI: 10.1016/j.ces.2013.02.045

Abstract (EN): Microtiter plates are one of the most widely used platforms for biofilm studies. However, these systems have been used rather blindly without knowledge of the flow characteristics inside them. Plates with 96-wells were used to study flow dynamics and its influence on Escherichia coli JM109(DE3) biofilm formation. Two different glucose concentrations (1 g L-1 and 0.25 g L-1) and two different shaking conditions were tested using incubators with orbital diameters of 50 and 25 mm. Biofilm growth was monitored for 60 h and the hydrodynamics were simulated by computational fluid dynamics. These simulations have shown that wall shear stress was higher near the air/liquid interface and average values of 0.070 and 0.034 Pa were obtained for the largest and smallest orbital diameter. For the high glucose concentration, the maximum biofilm amount was attained at 24 h and similar values were obtained in both incubators. For the low glucose concentration, lower values were attained. Numerical simulations indicated that microtiter plates can adequately model biofilm formation in relevant biomedical systems.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8