Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

duarte, aj (Autor) Outra; da silva, jcge (Autor) FCUP

Revista

Título: Sensors

Vol. 10 Nº 3

Páginas: 1661-1669

ISSN: 1424-3210

Editora: MDPI

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 13 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química > Quimíca inorgânica ; Ciências Físicas > Química > Química analítica > Espectroquímica

Outras Informações

ID Authenticus: P-003-910

DOI: 10.3390/s100301661

Abstract (EN): A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10-750 mu M with a limit of detection (LOD) of about 1 mu M. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9