Enzyme based assays in a sequential injection format: A review

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2011

Autores

Cristina I C Silvestre (Autor) Outra; Paula C A G Pinto (Autor) FFUP; Marcela A Segundo (Autor) FFUP; Lucia L M F S Saraiva (Autor) FFUP; Jose L F C Lima (Autor) FFUP

Revista

Título: Analytica Chimica Acta

Vol. 689 Nº 2

Páginas: 160-177

ISSN: 0003-2670

Editora: Elsevier

Indexação

ISI Web of Knowledge - 36 Citações

Scopus - 41 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-002-T76

DOI: 10.1016/j.aca.2011.01.048

Abstract (EN): Sequential injection analysis systems have been extensively exploited in the last decades for the implementation of enzyme based assays aiming the evaluation of enzyme activity or the determination of specific analytes. The most prominent aspects of the automation of enzymatic assays in these systems are discussed in this review. Special attention is devoted to the mode of enzyme manipulation in homogeneous or heterogeneous media and to the comparison with batch and flow injection enzyme methodologies. The possibility of implementing strategies for the enhancement of selectivity in specific determinations is also addressed. The more recent trends in this field are discussed focusing mainly on the miniaturization resorting to the lab-on valve platform as well as on the bead injection concept.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 18