Receptor-Mediated Gene Delivery Using PAMAM Dendrimers Conjugated with Peptides Recognized by Mesenchymal Stem Cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Jose L Santos (Autor) FCUP; Deepti Pandita (Autor) Outra; Joao Rodrigues (Autor) Outra; Ana P Pego (Autor) FEUP; Pedro L Granja (Autor) FEUP; Gary Balian (Autor) Outra; Helena Tomas (Autor) Outra

Revista

Título: Molecular Pharmaceutics

Vol. 7

Páginas: 763-774

ISSN: 1543-8384

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 93 Citações

Scopus - 95 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

ID Authenticus: P-003-76Y

DOI: 10.1021/mp9002877

Abstract (EN): As mesenchymal stem cells (MSCs) can differentiate into multiple cell types, the delivery of exogenous genes to this type of cell can be an important tool in tissue regeneration and engineering. However transfection of MSCs using nonviral gene delivery vectors is difficult, the development of more efficient and safe DNA vehicles being necessary. Moreover, specific transfection of MSCs may be required to avoid unwanted side effects in other tissues. In this study, a novel family of gene delivery vectors based on poly(amidoamine) (PAMAM) dendrimers functionalized with peptides displaying high affinity toward MSCs was prepared. The vectors were characterized with respect to their ability to neutralize, bind and compact plasmid DNA (pDNA). The complexes formed between the vectors and pDNA were analyzed concerning their size, zeta-potential, capacity of being internalized by cells and transfection efficiency. These new vectors exhibited low cytotoxicity, receptor-mediated gene delivery into MSCs and transfection efficiencies superior to those presented by native dendrimers and by partially degraded dendrimers.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12