Glutamate sol-gel amperometric biosensor based on co-immobilised NADP(+) and glutamate dehydrogenase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Gomes, SP (Autor) Outra; Dolezalova, J (Autor) Outra; Araujo, AN (Autor) FFUP; Couto, CMCM (Autor) Outra; Montenegro, MCBSM (Autor) FFUP

Revista

Título: Journal of Analytical Chemistry

Vol. 68

Páginas: 794-800

ISSN: 1061-9348

Editora: Pleiades Publishing

Indexação

ISI Web of Knowledge - 3 Citações

Scopus - 3 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-006-7VZ

DOI: 10.1134/s1061934813090049

Abstract (EN): In this work the preparation and electrochemical characterisation of a new glutamate amperometric sol-gel biosensor is described. A carbon paste electrode was electrochemically modified with the phenothiazine dye methylene green (MG). The NADP(+) and glutamate dehydrogenase were co-immobilised in a sol-gel matrix. When coupled to a flow injection system (FIA) the biosensor showed good electrocatalytic activity towards NADPH oxidation at the potential of + 0.3 V vs. Ag/AgCl; KCl sat., which represents a strong overpotential reduction. The biosensor yielded a linear response from 5.0 x 10(-5) to 1.0 x 10(-2) M glutamate concentration, with a detection limit of 5 x 10(-6) M and reproducibility of results better than 2.3% (RSD). Moreover, the implemented biosensor showed to be still useful after 7 months, 500 determinations.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7