Microbiology Laboratory

Code:

BIOL4103

Acronym:

BIOL4103

Level:

400

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2024/2025 - 1S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Cell and Molecular Biology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:BCM	16	Study plan since academic year 2024/2025	1	-	6	42	162

Teaching language

Portuguese
Obs.: Português - Suitable for English-speaking students

Objectives

Bibliography search and organization of a database.

Discussion of scientific papers.

Improve knowledge on microbiological techniques used commonly in nowadays research.

Develop capacities to conceive structure and put in place an experimental work/design.

Organization of results obtained in the experimental work, elaboration of a poster as for presentation in a scientific meeting.

Elaboration of a manuscript.

Learning outcomes and competences

Development of capacities in cultivation and molecular techniques in Microbiology

Working method

Presencial

Program

This course is mainly based on the planning and execution of a laboratorial study, treatment and analysis of the results and learning how to communicate them to the scientific community. This will be achieved by the preparation of a poster as for a scientific meeting and the elaboration of a manuscript. Culturing and genomic and/or proteomic molecular techniques will be performed in the laboratorial work. Several techniques frequently used in Microbiological studies namely Electron Microscopy, FISH, Brdu-FISH, CARD-FISH, MAR-CARD-FISH, Flow Cytometry, Fingerprinting Methods – DGGE, Pyrosequencing, q-PCR; real time-PCR and Microarrays will be studied focusing on their principles and applicability.

Mandatory literature

Reddy C. A., Beveridge T. J., Breznak J.A., Marzluf G., Schmidt T. M. & Snyder L.R; ; Methods for General and Molecular Microbiology, ASM Press, , 2007

Teaching methods and learning activities

Continuous evaluation during the course.

Poster for a communication in a conference (P)

Preparation of a scientific paper based on the laboratorial work developed (SP)

Oral presentation of a Microbiology topic (A)

NF = 30% (P) + 40% (SP) + 30% (A)

Evaluation Type

Distributed evaluation without final exam

Assessment Components

designation	Weight (%)
Apresentação/discussão de um trabalho científico	30,00
Trabalho escrito	70,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	120,00
Frequência das aulas	42,00
Total:	162,00

Eligibility for exams

not applicable

Calculation formula of final grade

Poster for a communication in a conference (P)

Preparation of a scientific paper based on the laboratorial work developed (SP)

Oral presentation of a Microbiology topic (A)

NF = 30% (P) + 40% (SP) + 30% (A)

Observations

Responsable for the discipline: Olga Maria Lage