Molecular and Cell Bioloy II
Keywords |
Classification |
Keyword |
OFICIAL |
Biology |
Instance: 2023/2024 - 2S ![Requerida a integração com o Moodle Ícone do Moodle](/fcup/pt/imagens/MoodleIcon)
Cycles of Study/Courses
Acronym |
No. of Students |
Study Plan |
Curricular Years |
Credits UCN |
Credits ECTS |
Contact hours |
Total Time |
L:B |
191 |
Official Study Plan |
1 |
- |
6 |
48 |
162 |
Teaching Staff - Responsibilities
Teaching language
Suitable for English-speaking students
Objectives
The Cell is the fundamental unit of life and its structure and functions are central to Biology. The Curricular Unit (UC) Cell Biology (BC) aims to provide the knowledge necessary to understand the architecture of the cell and the integrated functioning of cellular life. It is also the objective of this UC to work its contents in an application context, and to provide hands-on contact with different types of cells, organelles and cellular processes, as well as with some of the fundamental techniques and methodologies for the construction of the knowledge in Cell Biology (eg, optical microscopy and cell fractionation).
Learning outcomes and competences
The UC Cell Biology expects that the students acquire and develop the following knowledge, attitudes and skills:
- consistent knowledge about the general characteristics of the structure and ultrastructure of prokaryotic and eukaryotic cells;
- consistent knowledge about the major structural and functional aspects of all subcellular compartments and structures of the eukaryotic cell;
- consistent knowledge about the main cellular processes, namely transmembrane transport, communication and signalling, energy flow processes (chemiosmosis, glycolysis, photosynthesis, respiration), cell division, and programmed cell death;
- recognition of cell biology as a rapidly evolving cutting-edge area;
- critical spirit and curiosity towards the area of cell biology and to science in general;
- capacity to search and deepen topics directly or indirectly related to cell biology;
- capacity to interpret, interconnect and / or apply concepts of cell biology in varied contexts, namely in other fundamental and applied areas of life sciences (eg physiology, development, biotechnology, medicine);
- capacity to apply a critical and ethical attitude in the analysis of biological problems and their implications for society;
- basic communication skills in cell biology;
- capacity to work autonomously with an optical microscope;
- capacity to understand and correctly execute simple experimental methodologies of cell biology.
Working method
Presencial
Program
THEORETICAL PROGRAM
_____________________________________
Module I - The Cell and its studyThe cell as the basic unit of all living organisms. The prokaryotic and eukaryotic cell. Evolution of the cell. Techniques and methodologies for cell study: cell fractionation, cell isolation, cell and organ culture, optical and electron microscopy, immunological techniques,
in vivo methodologies, cell imaging.
Module II - Biological membranes and the social / environmental context of the cellLipids and carbohydrates: types,nomenclature and properties. Universal characteristics of biological membranes. Composition, architecture and dynamics of membranes. Transport of solutes through biological membranes. Types of transport molecules and their transport mechanisms. Social / environmental context of the cell: adherence and communication. Extracellular matrix and intercellular binding structures. Types of signals, mechanisms of perception and mechanisms of signal transduction. Receptors, molecular switches, secondary messengers and signaling complexes.
Module III - Structural and functional compartmentalization of the cellCell compartmentalization and intracellular protein sorting. Secretory or endomembrane system. Endoplasmic reticulum: structure and functions. Golgi apparatus: structure and functions. Synthesis, processing and transport of proteins, lipids and carbohydrates in the secretory system. Transit of membrane vesicles. Exocytosis, endocytosis and lysosomes. Peroxisomes: structure, functions and ontogeny. Cytoskeleton. Structure and function of actin filaments, microtubules and intermediate filaments. Motor proteins, cillia and flagella.
Module IV - Energy flow in the cellConservation and flow of energy in living beings. The central role of nucleotides in energy metabolism. Chemiosmotic synthesis of ATP. The role of membranes in bioenergetics. Photosynthesis. Plastids: general characteristics and plasticity. Capture of light energy. Photosystems. The thylakoid electron transport chain. The Z-scheme. Photophosphorylation. The carbon fixation cycle. Photorespiration. C4 photosynthesis and CAM photosynthesis. Glycolysis and fermentation. Aerobic respiration and anaerobic respiration. Mitochondria: general characteristics. The mitochondrial genome. The tricarboxylic acids cycle. Electron transport chain and oxidative phosphorylation. Diversity of central metabolic pathways. Classification of organisms regarding the source of carbon and the source of electrons.
Module V - The cell cycleLife cycle of prokaryotic and eukaryotic cells. Cell division in prokaryotic cells. Nucleus, nucleolus and other sub-nuclear structures. Chromosome organization and chromatin structure. Mitosis and meiosis. Regulation of the cell cycle. Programmed cell death.
PRACTICAL PROGRAM_________________________
I - The cells under the microscope. Observation of different cell types under the bright field optical microscope and of the respective ultrastructure in electron microscopy images.
II - Spectrophotometry in biochemistry. Spectrophotometric determination of a methylene blue solution.
III - Chloroplast isolation, chlorophyll quantification and study of the Hill reaction.
IV - The cell cycle and mitosis.
Mandatory literature
Alberts Bruce 070;
Molecular biology of the cell. ISBN: 9780815344643
Lodish Harvey F. 070;
Molecular cell biology. ISBN: 9781464109812
Complementary Bibliography
Nelson David L. David Lee 1942-;
Lehninger principles of biochemistry. ISBN: 978-1-57259-931-4
Salema Roberto;
Atlas de ultrastrutura celular
Azevedo Carlos 34040;
Biologia celular e molecular. ISBN: 978-972-757-354-7
Teaching methods and learning activities
Theoretical classes with a component of formal lecture and they may also have a component of interactive activities teacher/student and student/student (use of peer-based learning strategies), including the guided and discussed resolution of formative questionnaires, the use of case studies, and autonomous research work under guidance. Use of explanatory/power point classes recorded in video for autonomous study.
Practical lab classes including experimental planning, execution of practical procedures, and treatment and discussion of results.
keywords
Natural sciences > Biological sciences > Biology > Cell biology
Evaluation Type
Distributed evaluation without final exam
Assessment Components
designation |
Weight (%) |
Teste |
100,00 |
Total: |
100,00 |
Amount of time allocated to each course unit
designation |
Time (hours) |
Estudo autónomo |
114,00 |
Frequência das aulas |
48,00 |
Total: |
162,00 |
Eligibility for exams
Attendance in 75% of practical classes and realization of the practical test (TP).
Student workers need to do 2/3 of the practical classes work.
Calculation formula of final grade
The course assessment is made through tests.
The 1st test will be mid-term - T1. The 2nd test will be carried out on the date scheduled for the exam - T2. The tests will focus on the theoretical classes, and each one is rated on the 0-20 scale.
The tests have a minimum grade of 7,0 values. Only students who obtain a grade equal to or higher than 7,0 values can take the 2nd test.
An additional test on the pratical classes will take place towards the end of the semester (TP).
Final classification = [T1 + T2 + TP] / 3
In case of non-approval in the tests, it is possible to do a
global test on the date scheduled for the make up exam.
Classification improvement
Grade improvement can be made until the time of the make up exams of the academic year subsequent to the one in which the student obtained approval. It can therefore be done by tests (if taken during the normal exam period) or by a global test (if taken during the make up exam period).
Observations
Coordination: Mariana Sottomayor
Juri: Mariana Sottomayor and Luís Gustavo Pereira