Molecular Markers: Y chromosome

Code:

B4034

Acronym:

B4034

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2021/2022 - 2S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Forensic Genetics

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:GF	10	plano de estudos do Mestrado em Genética Forense a partir de 2013_2014.	1	-	6	42	162

Teaching language

Suitable for English-speaking students

Objectives

To master the use of Y chromosome (non-recombining) genetic markers in the forensic context

Learning outcomes and competences

mastering the use of Y chromosome specific genetic markers in forensic context

Working method

Presencial

Program

Evolution of sex chromosomes: differentiation and structure of Y chromosome
Y chromosome specific region; comparison with other primates: structure and evolution.
Y chromosome specific markers: STRs e SNPs.
Transmission properties of Y chromosome.
Usefulness of Y chromosome specific markers in paternity investigation and forensic identification: examples and likelihood ratio (LR) calculations
Y-STR databases: haplotype frequencies, sufficiency and sample size, population substructure
Y-STRs mutations - mechanisms and factors influencing Y-STRs mutation rate: number of repeats, type of mutation (gains and losses according to allele length), age.
Y chromosome genetic diversity in human populations: Y chromosome lineages phylogeny and worldwide distribution.
Male DNA of unknown provenance: ascertainment of population of origin through Y-STRs typing.

Mandatory literature

John M. Butler ; Forensic DNA typing. Biology, technology and genetics of STR markers
Gusmão L, Alves C ; Y chromosome STR typing. In Forensic DNA Typing Protocols, v.297 (Carracedo A., ed.), Humana Press Inc., Totowa, New Jersey, pp. 67-81.
Gusmão L, Brión M, Gomes I ; The human Y chromosome male specific polymorphisms and forensic genetics. In Handbook of Analytical separation. Vol. 6. Forensic Sciences. MJ Bogusz and R Smith (eds.). Elsevier Science. Amsterdam.
Karafet, T.M., et al. ; New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree. Genome Res, 18(5): p. 830-8.
Amorim A, Budowle B; Handbook of forensic genetics : biodiversity and heredity in civil and criminal investigation, World Scientific, 2016. ISBN: 9781786340771

Comments from the literature

https://yhrd.org/
http://www.fluxus-engineering.com/sharenet.htm

Teaching methods and learning activities

lectures and practical lessons

keywords

Natural sciences > Biological sciences > Biology > Genetics

Evaluation Type

Evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	100,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	120,00
Frequência das aulas	42,00
Total:	162,00

Eligibility for exams

participation in the lectures and practical lessons

Calculation formula of final grade

not applicable

Special assessment (TE, DA, ...)

not applicable

Classification improvement

not applicable

Observations

Coordinating Professor: Antonio Amorim