Molecular and Cellular Biology
Keywords |
Classification |
Keyword |
OFICIAL |
Biology |
Instance: 2021/2022 - 1S
Cycles of Study/Courses
Acronym |
No. of Students |
Study Plan |
Curricular Years |
Credits UCN |
Credits ECTS |
Contact hours |
Total Time |
L:EA |
9 |
The study plan from 2019 |
1 |
- |
6 |
48 |
162 |
Teaching language
Suitable for English-speaking students
Objectives
The Cell is the fundamental unit of life, and the knowledge of its physiology forms the foundation upon which all Biology as well as its applications. The objective of this UC is to make the student discover the Biology of the Cell at a global level, including the main structures and functional processes, and their respective molecular basis, as well as the experimental methodologies used to build that knowledge.
Learning outcomes and competences
The students should acquire the ability to understand, use, communicate and apply the basic concepts concerning the structure and functioning of cells, including their chemical composition, the role of membranes, the cell division, and the flow of information and energy. They should also acquire theoretical and practical skills in methods of study the cell, such as microscopy and the technology of recombinant DNA.
Working method
Presencial
Pre-requirements (prior knowledge) and co-requirements (common knowledge)
A prior knowledge is not necessary because this UC is basic in molecular and cellular biology. It may be a relevant this UC in terms of biological knowledge for those who, not being a Biology student, intend to take a master's degree in transversal areas with Biology, such as Plant Biotechnology, Bioinformatics….
Program
Flow of information. DNA organization in chromosomes and chromatin. Structure of DNA, nucleotides and nucleic acids. DNA duplication and repair mechanisms. Types of RNA and their function. RNA transcription and processing. Genetic code. Translation. Protein synthesis. Proteins: structure and function of proteins. Establishment of the three-dimensional structure “chaperones” Recombinant DNA technology, such as DNA extraction, Polymerase chain reaction (PCR).
Internal organization and function of the cell.
Biological membranes and their role in the cell / life. Membrane transport. Structure and functions of the secretory system: endoplasmic reticulum, Golgi complex and vesicle transit. Structure and functions of lysosomes and peroxisomes. Structure and function of the vacuole in plant cells. Structure and functions of the cytoskeleton in animal and plant cells.
Energy flow in cells and in the living world.
Breathing in the absence of oxygen: glycolysis and types of fermentation. Aerobic respiration and mitochondria structure and evolution. Chemosmotic theory and the role of membranes in energy conversion. Study cases. Photosynthesis and chloroplasts, structure and evolution of plastids. Case studies in agroforestry species.
Cell cycle and cell division. Checkpoints of cell division. The different phases of mitosis (division of the nucleus). Cytokinesis in plant and animal cells.
PRACTICAL LESSONS PROGRAM . Observation and measurement of different cell types under an optical microscope. Spectrophotometry and chlorophyll dosing. DNA extraction, PCR amplification, and analysis of the amplification products by agarose gel electrophoresis. Observation of mitosis in root apices of onion.
Mandatory literature
Bruce Alberts;
Essential cell biology. ISBN: 978-0-8153-2971-8
Complementary Bibliography
James Darnell;
Molecular cell biology. ISBN: 0-7167-1981-9
Gerald Karp;
Cell and molecular biology. ISBN: 978-0-470-04217-5
Teaching methods and learning activities
Theoretical lectures using powerpoint presentations and interaction with students using distance learning technologies, such as video-conferences and /or through the moodle platform. Practical laboratory classes carry out laboratory work. Stdents observe definitive and extemporaneous preparations under the microscope. Students handle some materials and get in tools used in biotechnology.
keywords
Natural sciences > Biological sciences > Biology > Molecular biology
Natural sciences > Biological sciences > Biology > Cell biology
Evaluation Type
Distributed evaluation with final exam
Assessment Components
designation |
Weight (%) |
Exame |
100,00 |
Total: |
100,00 |
Amount of time allocated to each course unit
designation |
Time (hours) |
Estudo autónomo |
114,00 |
Frequência das aulas |
48,00 |
Total: |
162,00 |
Eligibility for exams
Lectures are optional. Practical classes are obligatory with a limit of maximum 3 absences.
Calculation formula of final grade
The assessment will include the following components
1) Frequencies and/or final examination
Observations
Once again I emphasize that this discipline of Molecular and Cellular Biology should be part of the scientific culture of students who take the different courses at FCUP and intend to enter a master's degree that have to have some minimum of DNA; a DNA gene to protein, and flux of energy in living world.
Coordenadora da disciplina
Maria isabel Amorim