Reproduction in Arabidopsis: a Molecular Approach

Code:

BIOL4052

Acronym:

BIOL4052

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2015/2016 - 2S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Cell and Molecular Biology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:BCM	2	Official Study Plan until 2023/2024	1	-	6	42	162

Teaching language

English

Objectives

 

The main objectives of this course are to provide students with ability to handle all bio-informatics tools available to the plant that is considered a model in molecular biology.
Familiarize themselves with advanced processing plants, handling of single and double null mutants and mutants with genes labeled with different tags. Employ techniques of emasculation and pollination of plants, studies semi in vivo of the pollen tube growth and visualization of molecular markers by fluorescence microscopy and electronic microscopy. Evaluation of results of Real Time-PCR and evaluating the results of microarrays.

 

Learning outcomes and competences

The students will be able to go onto a scientific stay in any Molecular Biology lab, as well as they will have knowledge about Plants reproductive Biology.

Working method

Presencial

Program

 

The program of this course aims to acquaint the students with the current state of knowledge on the pollen pistil interactions in Arabidopsis, leading to double fertilization. Specifically, to study the signal molecules involved in the different parts of the pollen tube pathway into its target, the embryo sac; understanding the molecular mechanisms that may be present in these interactions and to learn the techniques that are used for these approaches. Students will be integrated into the lines of research of the academic staff involved, whose projects and scientific collaborations are related to the theme presented.
Students will also benefit from the material resources and equipment of the group, and from the knowledge already obtained and published in scientific journals.

 

Mandatory literature

Scott R. J. Rod J. 340; Molecular and cellular aspects of plant reproduction. ISBN: 9780521455251

Teaching methods and learning activities

 

Continuous evaluation during all the lab work, evaluating participation, punctuality, integration in the team, autonomy, motivation, organization of working time, initiative, rigour, method and organization skills.

Written report following research paper standards, of one of the lab works and an oral presentation from one of the focused subjects.

Evaluation Type

Distributed evaluation without final exam

Assessment Components

designation	Weight (%)
Participação presencial	30,00
Trabalho escrito	70,00
Total:	100,00

Calculation formula of final grade

Participation (up to 6 points) + Report (up to 14 points) = 20