Bio-imaging

Code:

B4045

Acronym:

B4045

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2013/2014 - 1S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Cell and Molecular Biology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:BCM	20	Official Study Plan until 2023/2024	1	-	6	42	162

Teaching language

Suitable for English-speaking students

Objectives

This course mainly techniques-based is designed: to provide a thorough understanding of modern microscopy technique; to present strategies in basic molecular biology technologies that enable the study of living cells with fluorescent proteins; and to provide essential knowledge how to use antibody technology to localise proteins.

Learning outcomes and competences

Upon successful completion of the course students should demonstrate to understand the application of different types of microscopes in cell biology and to identify and know how to use the increasingly sophisticated techniques that allow the study of current biological problems such as membrane dynamics, protein transport and cell signaling.

Working method

Presencial

Program

Introduction to light microscopy including specimen preparation; basic principles of optics. Fluorescence microscopy, confocal microscopy; image analysis. Modern microscopy techniques and basic molecular biology techniques in the study of cells. Antibody labeling vs. live cell imaging with fluorescent proteins (confocal microscopy of living cells); cell transformation with fluorescent protein fusion constructs. Intracellular trafficking, endomembrane compartmentalization and cell signalling. Photoconvertible fluorescent proteins. Electron microscopy, handling techniques of samples preparation and observation; correlative microscopy.

Mandatory literature

Chandler Douglas E.; Bioimaging. ISBN: 978-07637-3874-7

Teaching methods and learning activities

Lectures, seminars and laboratory work.

Evaluation Type

Distributed evaluation without final exam

Assessment Components

designation	Weight (%)
Trabalho escrito	80,00
Trabalho laboratorial	20,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	40,00
Frequência das aulas	50,00
Trabalho laboratorial	10,00
Total:	100,00

Calculation formula of final grade

Evaluation: Presentation and discussion of laboratory work results, 20%; Written laboratory report, 80%.