Molecular and Cellular Biology

Code:

B103

Acronym:

B103

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2012/2013 - 1S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Bachelor in Geology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
L:AST	0	Plano de Estudos a partir de 2008	3	-	5	-
L:CC	1	Plano de estudos de 2008 até 2013/14	3	-	5	-
L:F	0	Plano de estudos a partir de 2008	2	-	5	-
			3
L:G	3	P.E - estudantes com 1ª matricula anterior a 09/10	3	-	5	-
		P.E - estudantes com 1ª matricula em 09/10	3	-	5	-
L:M	0	Plano de estudos a partir de 2009	3	-	5	-
L:Q	7	Plano de estudos Oficial	3	-	5	-

Teaching language

Portuguese

Objectives

The students should acquire the ability to recognize, use, distribute and apply the basic concepts concerning the structure and functioning of cells, including their chemical composition, role of membranes, cell division, and flow of information and energy. They should also be acquired theoretical skills and practical experience in methods of study of the cell, such as microscopy and recombinant DNA technology

Learning outcomes and competences

.

Working method

Presencial

Program

1- The cell 1.1 - Cell versus life. Cell theory- from cellular anatomy to cell physiology. 1.2 - Cellular organization - prokaryotic cell and eukaryotic animal and plant. Cell dimensions. 1.3 - Evolution of the cell. 1.4 - Study of the cell. 1.4.1 - Optical microscopy. 1.4.2 – Fluorescence microscopy 1.4.3 -Confocal microscopy. 1.4.4 - Electron microscopy. 1.4.5 - Techniques of color, contrast and specific location - cytochemistry and immunocytochemistry. 2 - The chemistry of the cell / life 2.1 - Water. 2.2 - Carbohydrates. 2.3 - Lipids. 3 - The workers of the cell: structure and function of proteins 3.1 - Primary, secondary, tertiary and quaternary domains. 3.2 - Relationship structure / function - antibodies and enzymes. 3.3 - Regulation of the biological activity of a protein 3.4 - Establishment of three-dimensional structure: chaperones. 3.5 - Life Cycle: degradation by the ubiquitin pathway. 4 - Flow of information 4.1 - Nucleotides and nucleic acids. 4.2 - Structure of DNA-Watson and Crick model. 4.2 - Duplication and DNA repair mechanisms. 4.3 - Organization of DNA in chromosomes and chromatin. 4.4 - Types of RNA and its function. 4.5 - Transcription and RNA processing. 4.6 - Genetic code. 4.7 - Translation. 4.8 – The regulation of gene expression 4.9 - Mechanisms of genetic recombination. 4:10 - Transposons and viruses. 4.11 - Organization and evolution of the genome. 4.12 - Recombinant DNA Technology. 4.12.1 - Fragmentation, segregation, recombination and DNA sequencing. 4.12.2 - Cloning a DNA fragment. 4.12.3 - Polymerase chain reaction (PCR), variants and applications. 4.12.4 - Genetic Engineering and Biotechnology 5 - Organization and functioning of the internal cell 5.1 - The biological membranes and their role in cell / life. 5.2 - Transport across membranes 5.3 - Structure and functions of the cytoskeleton. 5.4 - Structure of the nucleus. 5.5 - Structure and functions of the secretory system, endoplasmic reticulum, Golgi Complex: vesicles and transit. 5.6 - Structure and function of lysosomes. 5.7 - Structure and functions of peroxisomes. 6 - Flow of energy in the cell 6.1 - Overview of flow of energy in cells and in the living world. 6.2 - Evolution of the processes involved in the energy flow in the living world. 6.3 - breathing in the absence of oxygen, glycolysis and types of fermentation. 6.4 - The chemiosmotic theory and the role of membranes in energy conversion. 6.5 - Photosynthesis and structure; diversity and evolution of plastids. 6.6 - Aerobic respiration and structure and evolution of mitochondria. 7 - Cell cycle and cell division 7.1-M phase: Mitosis. cytokinesis 7.2-Meiosis 7.3 - Regulation of cell cycle.

Mandatory literature

Azevedo, C. and Sunkel, C; Biologia Celular e Molecular, Lidel, 2012

Teaching methods and learning activities

Lectures using PowerPoint presentations and interaction with students. Laboratory classes "hands on".

keywords

Natural sciences > Biological sciences > Biology > Cell biology
Natural sciences > Biological sciences > Biology > Molecular biology

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Description	Type	Time (hours)	Weight (%)	End date
	Exame		90,00
	Trabalho laboratorial		10,00
	Total:	-	100,00

Amount of time allocated to each course unit

Description	Type	Time (hours)	End date
	Frequência das aulas
	Total:	0,00

Eligibility for exams

Lectures optional. Practical classes obligatory with a limit of fault Igua 3. Need to obtain use on the mini-tests and oral presentations made in the practical classes.

Calculation formula of final grade

METHODS OF ASSESSMENT The assessment will include the following components: 1) Frequency 1 and 2 with the theoretical and theoretical-practical or/ and a final exam that also includes theoretical and theoretical-practical. 2) Mini-test in practical classes. 3) Oral presentation of a work of practical lessons. PROCEDURES, CALCULATION OF FINAL GRADE: Final Grade = (2x + 1 x T P) / 3 Theoretical grade (T) A theoretical note may be obtained by two frequencies of theoretical material with a minimum grade of 4/10 for each one (I). If not achieved the minimum grade in some of them, the student is required to take the final exam of the theoretical matter (II). In case of approval on the frequencies, can be done only on the final exam time resource to improve their grade, being valid the highest note. I- Frequency 1 Frequency 2 T + T. Each frequency will be quoted for 10 (minimum score of 4 points) II - Final Exam quoted for 20 (minimum score of 8.0 points). Practical grade (P) Minitests practical classes and oral presentation (I) + Note theory and practice (II) = 20 points for price I - Mini-tests at the beginning of the practical lessons - 2 values. Oral presentation - 3 values. II - The theory and practice notes may be obtained by two frequencies of the theoretical and practical matters listed for 15 and with a minimum of 6 for each. If not achieved the minimum grade in some of them, the student is required to take the final exam of theoretical and practical matters. In case of approval on the frequencies, can be done for the final exam grade improvement, being valid the recourse examination. 

Observations

.