Recovery of ergosterol from Agaricus bisporus mushrooms via supercritical fluid extraction: A response surface methodology optimisation

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Almeida C.F. (Author) Other; Manrique Y.A. (Author) Other; Lopes J.C.B. (Author) Other; Martins, FG (Author) FEUP; Madalena M. Dias (Author) FEUP

Journal

Title: Heliyon

Vol. 10

ISSN: 2405-8440

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 1 Citation

Scopus - 1 Citation

Associated Projects

LA LSRE-LCM - Financiamento Plurianual 2020-2023 para a Unidade LA LSRE-LCM Laboratório de Processos de Separação e Reacção - Laboratório de Catálise e Materiais

Valor Natural - Valorização de Recursos Naturais através da Extração de Ingredientes de Elevado Valor Acrescentado para Aplicações na Indústria Alimentar

Other information

Authenticus ID: P-00Z-TCE

DOI: 10.1016/j.heliyon.2023.e21943

Abstract (EN): This work uses the response surface methodology to optimise ergosterol recovery from Agaricus bisporus mushrooms by supercritical fluid extraction. The influence of pressure, temperature, co-solvent use, and solvent-to-mushroom mass ratio was evaluated on yield and extract composition. Considering temperature, pressure, and co-solvent volume percentage, predictive models were regressed for extraction yield, ergosterol purity in the recovered fractions, and ergosterol recovery. The co-solvent volumetric fraction was the most influential factor, increasing the extraction yield and reducing the extract's ergosterol purity. A maximum ergosterol extract purity of (547.27 ± 2.37) mgergosterol·gextract¿1 was obtained at a pressure of 100 bar, temperature of 50 °C and co-solvent volume percentage of 5 % v/v. The highest ergosterol recovery was (6.23 ± 0.06) mgergosterol·gdw¿1 for operating conditions of 244 bar, 56 °C, and 8 % v/v of co-solvent. Coefficients of determination close to one were obtained for all models, indicating good agreement with experimental data.

Language: English

Type (Professor's evaluation): Scientific