Quantification of alpha-lactalbumin in human milk: Method validation and application

• Type: Another Publication in an International Scientific Journal
• Date: 2007

Authors

Lucia H L M L M Santos (Author) Other; Isabel M P L V O Ferreira (Author) FFUP

Journal

Title: Analytical Biochemistry

Vol. 362

Pages: 293-295

ISSN: 0003-2697

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 5 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-B5C

DOI: 10.1016/j.ab.2006.12.002

Abstract (EN): A reversed-phase high-performance liquid chromatography method with ultraviolet detection was optimized and validated for quantification of ¿-lactalbumin in human milk. It was found that the analysis of ¿-lactalbumin can be performed by semiquantitative methods, such as electrophoresis. Samples were skimmed by centrifugation at 5000 rpm, at room temperature, for 5 min, filtered using Whatman No. 42, and diluted 1/5 with deionized water. The external standard method was used to calibrate the system for ¿-lactalbumin quantification. The precision of the method taking into account its repeatability and reproducibility was evaluated. The results show that the method was able to quantify ¿-lactalbumin of human milk with good precision and shorter analysis time.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3