Cell cultures II - Animals

Code:

BIOL4060

Acronym:

BIOL4060

Level:

400

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2024/2025 - 1S

Active?	Yes
Responsible unit:	Department of Biology
Course/CS Responsible:	Master in Applications in Biotechnology and Synthetic Biology

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
M:ABBS	6	The study plan from 2019/20	1	-	3	21	81

Teaching language

Portuguese and english

Objectives

Provide students with basic knowledge of in vitro animal cell cultures, practical applications and cryopreservation techniques. Provide students with basic knowledge of cellular manipulation in aseptic conditions.

Learning outcomes and competences

At the end of the course, the student should be able to:

- recognize the various techniques of in vitro 2D and 3D animal cultures

- recognize the basic principles of animal cell biology in culture

- execute methods used in animal cell cultures and assessment of cell growth and viability

- recover cryopreserved cells and cryopreserve cells

Working method

Presencial

Pre-requirements (prior knowledge) and co-requirements (common knowledge)

n.a.

Program

Animal cell and tissue cultures. Historical references. Media and culture conditions. Biology of the cell in culture. Characterization of the culture (growth curves, confluence) and sub culturing. Stem cells, embryonic and adult cells. Plasticity. Cell immortalization and cell lines. Cryopreservation. Cultures in 2D and 3D. Applications and manipulation of animal cells in different scientific fields.

Mandatory literature

vários autores; artigos e capítulos de livro específicos

Teaching methods and learning activities

Theoretical and practical classes.

The practical classes are compulsory.

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	75,00
Participação presencial	25,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Estudo autónomo	57,00
Frequência das aulas	21,00
Apresentação/discussão de um trabalho científico	3,00
Total:	81,00

Eligibility for exams

Attendance of 2/3 of the classes

Calculation formula of final grade

Final grade: final exam covering theory and practical subjects graded to 15 points and oral presentation of a program subject graded to 5 points

Final exam minimum mark - 6 points

Classification improvement

Classification improvement only in the final exam (FE) component.

Observations

Coordinator Professor: Raquel Almeida