Microbiology of foods: from preservation to safety
| Keywords |
| Classification |
Keyword |
| OFICIAL |
Biology |
Instance: 2021/2022 - 1S 
Cycles of Study/Courses
Teaching language
Portuguese
Objectives
The course should grant the students with the knowledge needed to understand the importance of food microbiology and its applications in the area of training in Food Science and Technology.
The aim is to understand interactions between microorganisms and food within a food safety perspective.
The contents of this course are organized in order to enable students to understand the importance of microorganisms as etiological agents of disease, as agents of spoilage, and as agents of production and/or preservation of food. The laboratory program aims to familiarize students with the main methodologies and microbiological criteria used in microbiological analysis of water, food products, surfaces and food handlers.
Learning outcomes and competences
This course should grant the students with the knowledge needed to enable them with the adequate competencies to intervene in a broad range of issues related with food microbiology, namely:
- understand the extrinsic and intrinsic factors modulating the growth of microorganisms in foods, and recruit the acquired knowledge to plan and propose suitable food preservation methods.
- acknowledge the importance of predictive microbiology as a tool to infer the growth or inactivation of microorganisms in foods.
- be familiar with the main foodborne pathogens: epidemiology; risky foods; incubation; disease symptoms; prevention and detection.
- be familiar with the main food spoilers microorganisms and detection methods.
- be acquainted with key international directives concerning microbiological safety of foodstuffs.
Working method
Presencial
Pre-requirements (prior knowledge) and co-requirements (common knowledge)
General knowledge of Microbiology, Biology, Biochemistry, Safety and Hygiene at Work and Laboratory Quality Control.
Program
Theoretical classes
I. Microorganisms in food: food spoilage, food-borne diseases, food production and bio-preservation.
Microbial contamination sources in food.
II. Limiting factors of microbial growth in food
III. Microbial diseases transmitted by water/food.
IV. Microbiological analysis of food products and water for human consumption. Microbiological analysis of surfaces and food handlers.
V. International food regulation. The Codex Alimentarius Comission. ISO Standards. HACCP system.
Laboratory classes
- Microbiological analyses of food. Collection and preparation of samples. Methods for enumeration, detection and identification. Total plate count. Bioindicator microorganisms. Pathogenic microorganisms.
- Microbiological criteria.
- Microbiological analysis of drinking water. Microbiological control of drinking water. Criteria and standards of quality of drinking water.
- Antimicrobial properties of spices.
- Microbiological analysis of surfaces and handlers.
- Alternative methods for microbial detection
- Application of molecular biology techniques for the detection / identification of microorganisms in food samples.
Mandatory literature
Forsythe S.J. ; The Microbiology of Safe Food, 2000
Doyle Michael P. 340;
Food microbiology. ISBN: 978-1-55581-208-9
Yousef Ahmed Elmeleigy;
Food microbiology. ISBN: 978-0-471-39105-0
Banwart George J.;
Basic food microbiology
Complementary Bibliography
Jay, Loessner & Golden; Modern Food Microbiology, 2005
Hoorfar J. (Jeffrey) 340;
Rapid detection, characterization, and enumeration of foodborne pathogens. ISBN: 9781555815424
Teaching methods and learning activities
This course is organized in theoretical and practical classes.
Expository and demonstrating methodologies, as well as problem-solving, will be the main teaching strategies.
Lectures will be given by themes, with audiovisual resources. Emphasis will be given to the practical application of theoretical concepts of Food Microbiology, encouraging their interconnection with laboratory classes. Practical classes will be performed in a laboratory, in order to teach the main techniques applied for microbiological analysis of food, water, surfaces and food handlers.
Evaluation Type
Distributed evaluation with final exam
Assessment Components
| designation |
Weight (%) |
| Exame |
75,00 |
| Trabalho escrito |
25,00 |
| Total: |
100,00 |
Amount of time allocated to each course unit
| designation |
Time (hours) |
| Apresentação/discussão de um trabalho científico |
20,00 |
| Estudo autónomo |
100,00 |
| Frequência das aulas |
24,00 |
| Trabalho laboratorial |
18,00 |
| Total: |
162,00 |
Eligibility for exams
Attendance at the UC is conditioned to the presence of the student in 75% of the total number of laboratory classes provided.
Calculation formula of final grade
Final classification obtained from theoretical and practical evaluations.
CF = (0.5 x NT) + (0.25 x NTP) + (0.25 x NS)
Theoretical component (NT) - final written exam (minimum score 10.0 points)
A seminar (oral presentation of a work) - 25% (NS)
Practical component evaluation - theoretical and practical final exam - 25% (NTP)
Only students who obtain grade equal to or greater than 9.5 values will be approved.