Palmitic Acid and Ergosta-7,22-dien-3-ol Contribute to the Apoptotic Effect and Cell Cycle Arrest of an Extract from Marthasterias glacialis L. in Neuroblastoma Cells

• Type: Article in International Scientific Journal
• Date: 2014

Authors

David M Pereira (Author) FFUP; Georgina Correia da Silva (Author) FFUP; Patricia Valentao (Author) FFUP; Natercia Teixeira (Author) FFUP; Paula B Andrade (Author) FFUP

Journal

Title: Marine Drugs

Vol. 12

Pages: 54-68

ISSN: 1660-3397

Publisher: MDPI

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 33 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-009-3G7

DOI: 10.3390/md12010054

Abstract (EN): We describe the effect of a chemically characterized lipophilic extract obtained from Marthasterias glacialis L. against human breast cancer (MCF-7) and human neuroblastoma (SH-SY5Y) cell lines. Evaluation of DNA synthesis revealed that both cell lines were markedly affected in a concentration-dependent way, the SH-SY5Y cell line being more susceptible. Cell cycle arrest was observed, an effect induced by the sterol, ergosta-7,22-dien-3-ol, present in the extract. Morphological evaluation of treated cells showed the advent of lipid droplets and chromatin condensation compatible with apoptosis, which was confirmed by the evaluation of caspase-3 and -9 activities. Palmitic acid was the main compound responsible for this apoptotic effect by a ceramide-independent mechanism that involved endoplasmic reticulum (ER)-stress with upregulation of CCAAT/-enhancer-binding protein homologous protein (CHOP).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15