NMR study of the interaction of fluorescent 3-hydroxy-4-pyridinone chelators with DMPC liposomes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Moniz, T (Autor) Outra; de Castro, B (Autor) FCUP; Maria Rangel (Autor) ICBAS; Ivanova, G (Autor) Outra

Revista

Título: Physical Chemistry Chemical Physics

Vol. 18

Páginas: 5027-5033

ISSN: 1463-9076

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-00K-52S

DOI: 10.1039/c5cp05273d

Abstract (EN): In the present study we discuss the interaction of two fluorescent 3-hydroxy-4-pyridinone chelators (MRB7 and MRB8) of different lipophilicities with DMPC liposomes based on the analysis of the shifts of the resonance NMR signals and changes in the translational diffusion of both species. The analysis of the variation of the resonance signals of the chelators indicates that both MRB7 and MRB8 strongly interact with the liposomes and that such interaction occurs through both the fluorophore and the chelating moieties of the chelator's framework. Analysis of the variations in the characteristic resonance signals of the lipid provides evidence that MRB7 is able to reach the hydrophobic zone of the bilayer independent of the chelator concentration. The present results corroborate the fact that ethyl substituents in the amino groups of the xanthene ring and the thiourea link are important for the chelator's ability to diffuse across the lipid bilayer.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7