Modeling and simulation of protein adsorption in permeable chromatographic packings: a double linear driving force model

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Leitao, A (Author) Other; Alírio Rodrigues (Author) FEUP

Journal

Title: Biochemical Engineering Journal

Vol. 3 No. 2

Pages: 131-139

ISSN: 1369-703X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-001-4NE

DOI: 10.1016/s1369-703x(99)00011-x

Abstract (EN): Permeable large-pore packings have many applications, particularly in perfusion chromatography for bioseparations. The major objectives of this paper are: (i) to develop a double linear driving force (LDF) model based on linear driving force approximations inside throughpores and microparticles to simulate dynamic nonlinear adsorption in columns having spherical permeable chromatographic packings with a bidisperse pore structure; and (ii) to analyze the performance of this simple model to simulate breakthrough and elution curves for bovine serum albumin adsorption on POROS Q/M large-pore particles (PerSeptive Biosystems, Cambridge, MA, USA), in comparison with a more realistic and difficult model (intraparticle diffusion/convection model) which is also presented in this paper. Model results obtained for several values of the intraparticle Peclet number, lambda, resulting from the convective flow in the throughpores show the very reasonable performance of the double LDF model in simulating breakthrough and elution curves of proteins, particularly for higher values of lambda. This finding is of relevance due to the remarkable saving in computation time afforded with the double LDF model.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9