The anabaena sp. PCC 7120 exoproteome: Taking a peek outside the box

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Oliveira, P (Author) Other; Martins, NM (Author) Other; Santos, M (Author) Other; Couto, NAS (Author) Other; Wright, PC (Author) Other; Tamagnini, P (Author) FCUP

Journal

Title: Life

Vol. 5

Pages: 130-163

ISSN: 0024-3019

Publisher: MDPI

Indexing

ISI Web of Knowledge

Scopus - 41 Citations

Pubmed / Medline

Scientific classification

CORDIS: Natural sciences > Biological sciences > Biology > Microbiology

Other information

Authenticus ID: P-00A-8EQ

DOI: 10.3390/life5010130

Abstract (EN): The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp. PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as ¿hypothetical¿, falls into the ¿other categories¿ set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyanobacterial exoproteome studies and focus on the potential of exploring cyanobacteria as cell factories to produce and secrete selected proteins. © 2015 by the authors; licensee MDPI, Basel, Switzerland.

Language: English

Type (Professor's evaluation): Scientific