On-plate digestion using a commercial microfraction collector for nano-HPLC matrix-assisted laser desorption/ionization tandem time-of-flight protein analysis

• Type: Another Publication in an International Scientific Journal
• Date: 2008

Authors

vitorino, r (Author) Other; guedes, s (Author) Other; tomer, k (Author) Other; domingues, p (Author) Other; duarte, j (Author) FADEUP; amado, f (Author) Other

Journal

Title: Analytical Biochemistry

Vol. 380 No. 1

Pages: 128-130

ISSN: 0003-2697

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-WAK

DOI: 10.1016/j.ab.2008.05.006

Abstract (EN): A new method for on-plate protein digestion and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry analysis is proposed involving an automated one-step sample separation using nano-flow HPLC followed by nanoliter fraction collection and on-plate digestion with trypsin. This procedure uses a commercial automatic nanoliter fraction collection system for on-line spotting of the eluent onto a MALDI target. After protein digestion, the reaction is stopped by the addition of acidified matrix using the same automated system. Collected spots are subsequently analyzed using a MALDI tandem time-of-flight (TOF/TOF) mass spectrometer for protein sequencing and identification.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3