Trimipramine determination in pharmaceutical preparations with an automated multicommutated reversed-flow system

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Prior, JAV (Author) Other; Santos, JLM (Author) FFUP; Lima, JLFC (Author) FFUP

Journal

Title: Journal of Pharmaceutical and Biomedical Analysis

Vol. 33 No. 5

Pages: 903-910

ISSN: 0731-7085

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

ISI Web of Science

Scopus - 14 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-000-E4T

DOI: 10.1016/s0731-7085(03)00379-0

Resumo (PT): In this work an automated multicommutated flow methodology was implemented for the spectrophotometric determination of trimipramine in pharmaceutical preparations by oxidation with ammonium monovanadate in acidic medium. The developed procedure exploits a new approach for sample/reagent intermixing by combining binary sampling with flow-reversal. Rather than inserting the sample as a single continuous volume the intercalation of multiple small sample and reagent aliquots, under a time-based control, created multiple reaction interfaces that promoted reaction zone homogenisation even in limited dispersion conditions. Additionally, the reaction interfaces were reversed, increasing mutual zone penetration, which contributed to a faster reaction development while assuring a low dispersion pattern. A linear range of determination was verified for trimipramine concentrations between 1.0 and 18.0 μg ml−1 with a relative standard deviation (n=10) lower than 1.69% and a sample throughput of about 26 samples per hour. The results were in agreement with those obtained by the reference procedure with relative deviations lower then 2.37%. <br> <br> Keywords: Trimipramine; Multicommutated flow system; Reversed flow; Binary sampling; Spectrophotometry <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TGX-496NRVX-2&_user=2460038&_coverDate=12%2F05%2F2003&_rdoc=6&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235266%232003%23999669994%23473113%23FLA%23display%23Volume)&_cdi=5266&_sort=d&_docanchor=&_ct=39&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=738eb85bc5c3fc1b52e8c9db33d71141"> Texto integral</a> <br> <br>

Abstract (EN): In this work an automated multicommutated flow methodology was implemented for the spectrophotometric determination of trimipramine in pharmaceutical preparations by oxidation with ammonium monovanadate in acidic medium. The developed procedure exploits a new approach for sample/reagent intermixing by combining binary sampling with flow-reversal. Rather than inserting the sample as a single continuous volume the intercalation of multiple small sample and reagent aliquots, under a time-based control, created multiple reaction interfaces that promoted reaction zone homogenisation even in limited dispersion conditions. Additionally, the reaction interfaces were reversed, increasing mutual zone penetration, which contributed to a faster reaction development while assuring a low dispersion pattern. A linear range of determination was verified for trimipramine concentrations between 1.0 and 18.0 mug ml(-1) with a relative standard deviation (n = 10) lower than 1.69% and a sample throughput of about 26 samples per hour. The results were in agreement with those obtained by the reference procedure with relative deviations lower then 2.37%.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8