A procedure to harmonize the hydrodynamic force during microbial cultivation in shaking flasks

• Type: Article in International Scientific Journal
• Date: 2023-09-19

Authors

Inês Gomes (Author) FEUP; Isabel M. Oliveira (Author) Other; Anabela Borges (Author) FEUP; Simões M (Author) FEUP; Lúcia C. Simões (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION

Vol. 24

ISSN: 1935-7877

Indexing

ISI Web of Knowledge - 0 Citations

ISI Web of Science

Scopus - 0 Citations

Associated Projects

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

GermIrrad - Sistema automatizado de irradiação germicida

Other information

Authenticus ID: P-00Z-8X1

DOI: 10.1128/jmbe.00099-23

Abstract (EN): Shake flask cultivation is a routine technique in microbiology and biotechnology laboratories where cell growth can be affected by the hydrodynamic conditions, which depend on the agitation velocity, shaking diameter, and shake flask size. Liquid agitation is implemented inherently to increase aeration, substrate transfer to the cells, and prevent sedimentation, disregarding the role of hydrodynamics in microbial growth and metabolism. Here, we present a simple approach to help standardize the hydrodynamic forces in orbital shakers to increase the experimental accuracy and reproducibility and give students a better knowledge of the significance of the agitation process in microbial growth.

Language: English

Type (Professor's evaluation): Educational

No. of pages: 4