Leishmania Vesicle-Depleted Exoproteome: What, Why, and How?

• Type: Another Publication in an International Scientific Journal
• Date: 2022

Authors

Esteves, S (Author) Other; Costa, I (Author) Other; Luelmo, S (Author) Other; Santarem, N (Author) Other; Anabela Cordeiro da Silva (Author) FFUP

Journal

Title: Microorganisms

Vol. 65

Final page: 2435

Publisher: MDPI

Indexing

ISI Web of Knowledge - 0 Citations

Other information

Authenticus ID: P-00X-SCT

DOI: 10.3390/microorganisms10122435

Abstract (EN): Leishmaniasis, a vector-borne parasitic protozoan disease, is among the most important neglected tropical diseases. In the absence of vaccines, disease management is challenging. The available chemotherapy is suboptimal, and there are growing concerns about the emergence of drug resistance. Thus, a better understanding of parasite biology is essential to generate new strategies for disease control. In this context, in vitro parasite exoproteome characterization enabled the identification of proteins involved in parasite survival, pathogenesis, and other biologically relevant processes. After 2005, with the availability of genomic information, these studies became increasingly feasible and revealed the true complexity of the parasite exoproteome. After the discovery of Leishmania extracellular vesicles (EVs), most exoproteome studies shifted to the characterization of EVs. The non-EV portion of the exoproteome, named the vesicle-depleted exoproteome (VDE), has been mostly ignored even if it accounts for a significant portion of the total exoproteome proteins. Herein, we summarize the importance of total exoproteome studies followed by a special emphasis on the available information and the biological relevance of the VDE. Finally, we report on how VDE can be studied and disclose how it might contribute to providing biologically relevant targets for diagnosis, drug, and vaccine development.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 19