Red seaweeds strengthening the nexus between nutrition and health: phytochemical characterization and bioactive properties of Grateloupia turuturu and Porphyra umbilicalis extracts

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Ferreira, J (Author) Other; Hartmann, A (Author) Other; Martins Gomes, C (Author) Other; Nunes, FM (Author) Other; Souto, EB (Author) Other; Santos, DL (Author) Other; Abreu, H (Author) Other; Pereira, R (Author) Other; Pacheco, M (Author) Other; Gaivao, I (Author) Other; Silva, AM (Author) Other

Journal

Title: Journal of Applied Phycology

Vol. 33

Pages: 3365-3381

ISSN: 0921-8971

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 0 Citations

Other information

Authenticus ID: P-00V-77X

DOI: 10.1007/s10811-021-02529-6

Abstract (EN): The red seaweeds Grateloupia turuturu and Porphyra umbilicalis are commonly used as human food, mainly in East Asia, being valuable nutritional sources. However, studies focusing on their composition and bioactivities are scarce. Thus, G. turuturu and P. umbilicalis hydroethanolic and water (infusion and decoction) extracts were prepared to characterize their bioactive compounds; assess antioxidant activity against ABTS(center dot+), (OH)-O-center dot and (NO)-N-center dot radicals and evaluate anti-proliferative and anti-inflammatory activities using mouse macrophage (RAW 264.7) cells. Folin-Ciocalteau results showed that G. turuturu and P. umbilicalis water extracts have fourfold and threefold, respectively, higher content in reducing compounds than hydroethanolic extracts, with the water decoctions on the richer ones (15.7 +/- 0.3 and 11.9 +/- 0.2 mg GAE g(-1) dw, respectively; p<0.05). In the same way, decoction extracts of both seaweeds showed the highest antioxidant activity, measured as ABTS(center dot+) scavenging (49.4 +/- 1.6 and 46.1 +/- 1.8 mmol TE g(-1) dw, for G. turuturu and P. umbilicalis, respectively; p<0.05). Concerning (OH)-O-center dot radical, both hydroethanolic extracts exhibited identical (p>0.05) production inhibition capacity (similar to 55%), however higher than water extracts (p<0.05). Grateloupia turuturu water extracts produced the highest inhibition capacity against (NO)-N-center dot production, 54-56%. RP-HPLC-DAD analysis revealed the presence of mycosporine-like amino acids (MAAs) shinorine, palythine, porphyra-334 and asterina-330 identified in both seaweeds. All extracts produced dose- and time-dependent anti-proliferative activity, being P. umbilicalis infusion extracts the most active (IC50=0.43 mg mL(-1)). At non-cytotoxic concentrations, dose-dependent anti-inflammatory activity was observed for all extracts, highlighting the P. umbilicalis hydroethanolic extract at 0.2 mg mL(-1) that inhibited similar to 55% (of control) of lipopolysaccharide-induced NO release. In conclusion, G. turuturu and P. umbilicalis are rich in bioactive compounds, in particular MAAs, responsible for the bioactivities here reported, reinforcing their potential as functional food as well as sources of bioactive compounds to food and pharmaceutical industries.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17