Analytical tools for urocanic acid determination in human samples: A review

• Type: Another Publication in an International Scientific Journal
• Date: 2021

Authors

Lima, FV (Author) Other; Martins, TEA (Author) Other; Morocho Jacome, AL (Author) Other; Almeida, IF (Author) FFUP; Rosado, CF (Author) Other; Velasco, MVR (Author) Other; Baby, AR (Author) Other

Journal

Title: Journal of Separation Science

Vol. 44 No. 16

Pages: 438-447

ISSN: 1615-9306

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-00T-011

DOI: 10.1002/jssc.202000713

Abstract (EN): Urocanic acid is a chromophore found in the skin that has been identified as an important immunosuppressant and carcinogenesis mediator through its photoisomerization from trans to cis form induced by ultraviolet radiation. Research on analytical methods that explore urocanic acid isomerization is indispensable to fully understand the deleterious effects mediated by this biomarker. In this context, the current relevant analytical methods for determination of these isomers in human samples are summarized in this review. The methods presented here are applicable to human samples collected by noninvasive methods (or minimally invasive), encompassing an array of analytical techniques, including high-performance capillary electrophoresis, confocal Raman spectroscopy, gas chromatography, high-performance liquid chromatography, and mass spectrometry, among others. Developed high-performance liquid chromatography methods have proven to be advantageous, allowing noninvasive collections for in vivo analysis and the confocal Raman, specially, for real-time analysis. Among all these methods, high-performance liquid chromatography is the most investigated one with mass spectrometry or ultraviolet detector, and the mass spectrometry detector being the most studied in the last years, demonstrating high sensitivity, very low detection limits, and accurate identification, especially for clinical investigations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10