Antibiofilm activity of glycolic acid and glyoxal and their diffusion–reaction interactions with biofilm components

• Type: Article in International Scientific Journal
• Date: 2022-02-01

Authors

Inês B. Gomes (Author) FEUP; Susana Fernandes (Author) FEUP; Manuel Simões (Author) FEUP

Journal

Title: Food Research International

Vol. 152 No. Art. 110921

Pages: 1-12

ISSN: 0963-9969

Publisher: Elsevier

Indexing

ISI Web of Knowledge

ISI Web of Science

Scopus

Associated Projects

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Biocide_for_Biofilm - Desenvolvimento de novas soluções biocidas para o controlo efetivo de biofilmes

GermIrrad - Sistema automatizado de irradiação germicida

Other information

DOI: 10.1016/j.foodres.2021.110921

Abstract (EN): Biofilms on food-contact surfaces act as potential reservoirs of microbial pathogens and can cause operational problems. The search for effective biofilm control agents is a significant research need. In this study, glycolic acid (GA) and glyoxal (GO) were tested in the control of biofilms formed by Bacillus cereus and Pseudomonas fluorescens. Benzalkonium chloride (BAC) and peracetic acid (PAA) were used as reference biocides for industrial surface disinfection. The action of the selected biocides was assessed in bacterial motility, culturability, biofilm removal and inactivation, interference with biofilm components and limitation of biocide penetration through the biofilms (reaction-diffusion interactions). Bacterial motility was not affected by the exposure to subinhibitory biocide concentrations. In terms of antibiofilm activity, B. cereus biofilms were tolerant to the action of BAC, GA, and GO, with reductions of circa 2-log CFU/cm(2). Even 10000 mu g/mL of PAA had modest effects against B. cereus biofilms (5-log CFU/cm(2)). On the other hand, P. fluorescens biofilms were more susceptible to the biocides, except BAC which was not effective. The minimum concentrations to cause 3-log CFU/cm(2) reduction in P. fluorescens biofilms were 10000 mu g/mL of PAA and GA, and 20000 mu g/mL of GO. GO and BAC were the most actives for biofilm removal, while high biofilm inactivation was caused by GA and PAA. In general, biofilm components affected the antimicrobial activity of all the biocides. The activity of GA and GO was not notably diffusion-reaction limited in contrast to PAA. Overall, this study demonstrated that GA and GO had potential antibiofilm activity, being limited at a low level by physicochemical interactions with biofilm components.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12