Antimicrobial Susceptibility of Persister Biofilm Cells of Bacillus cereus and Pseudomonas fluorescens

• Type: Article in International Scientific Journal
• Date: 2022-01-13

Authors

Inês B. Gomes (Author) FEUP; Susana Fernandes (Author) FEUP; Manuel Simões (Author) FEUP; Sérgio Filipe Sousa (Author) FMUP

Journal

Title: Microorganisms

Vol. 10 No. 1

Pages: 1-12

Publisher: MDPI

Indexing

ISI Web of Knowledge - 1 Citation

ISI Web of Science

Scopus - 1 Citation

Associated Projects

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Biocide_for_Biofilm - Desenvolvimento de novas soluções biocidas para o controlo efetivo de biofilmes

ALGAVALOR - MicroALGAs: produção integrada e VALORização da biomassa e das suas diversas aplicações

GermIrrad - Sistema automatizado de irradiação germicida

Other information

Authenticus ID: P-00W-0DM

DOI: 10.3390/microorganisms10010160

Abstract (EN): The present study evaluates the antimicrobial susceptibility of persister cells of Bacillus cereus and Pseudomonas fluorescens after their regrowth in suspension and as biofilms. Two conventional (benzalkonium chloride-BAC and peracetic acid-PAA) and two emerging biocides (glycolic acid-GA and glyoxal-GO) were selected for this study. Persister cells resulted from biofilms subjected to a critical treatment using the selected biocides. All biocide treatments developed B. cereus persister cells, except PAA that effectively reduced the levels of vegetative cells and endospores. P. fluorescens persister cells comprise viable and viable but non-culturable cells. Afterwards, persister cells were regrown in suspension and in biofilms and were subjected to a second biocide treatment. In general, planktonic cultures of regrown persister cells in suspension lost their antimicrobial tolerance, for both bacteria. Regrown biofilms of persister cells had antimicrobial susceptibility close to those regrown biofilms of biocide-untreated cells, except for regrown biofilms of persister P. fluorescens after BAC treatment, which demonstrated increased antimicrobial tolerance. The most active biocide against persister cells was PAA, which did not promote changes in susceptibility after their regrowth. In conclusion, persister cells are ubiquitous within biofilms and survive after critical biocide treatment. The descendant planktonic and biofilms populations showed similar properties as the original ones.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12