An Improved LC-MS/MS Method for the Analysis of Thirteen Cytostatics on Workplace Surfaces

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Portilha Cunha, MF (Author) Other; Ramos, S (Author) Other; Silva, Adrian (Author) FEUP; Norton, P (Author) Other; Arminda Alves (Author) FEUP; Mónica Santos (Author) FEUP

Journal

Title: Pharmaceuticals

Vol. 14

Final page: 754

ISSN: 1424-8247

Publisher: MDPI

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 4 Citations

Other information

Authenticus ID: P-00V-C9H

DOI: 10.3390/ph14080754

Abstract (EN): Cytostatics are drugs used in cancer treatment, which pose serious risks to healthcare workers. Dermal absorption via surface contamination is the key exposure route; thus, rapid, reliable, and validated analytical methods for multicomponent detection are crucial to identify the exposure risk. A surface-wipe-sampling technique compatible with hospitals' safety requirements (gauze, 1 mL isopropanol) and a fast and simple extraction method (1 mL acetonitrile, 20 min ultrasonic bath, evaporation, reconstitution in 200 mu L acetonitrile), coupled with liquid chromatography-tandem mass spectrometry analysis, were developed. It allowed identification and quantification of 13 cytostatics on surfaces: cyclophosphamide, doxorubicin, etoposide, ifosfamide, paclitaxel, bicalutamide, capecitabine, cyproterone, flutamide, imatinib, megestrol, mycophenolate mofetil, prednisone. Good linearity, sensitivity, and precision were achieved (R-2 > 0.997, IDLs < 4.0 pg/cm(2), average CV 16%, respectively). Accuracy for four model surfaces (melamine-coated wood, phenolic compact, steel 304, steel 316) was acceptable (80 +/- 12%), except for capecitabine and doxorubicin. Global uncertainty is below 35% for concentrations above 100 pg/cm(2) (except for capecitabine and doxorubicin)-a guidance value for relevant contamination. Method application in a Portuguese university hospital (28 samples) identified the presence of seven cytostatics, at concentrations below 100 pg/cm(2), except for three samples. The widespread presence of cyclophosphamide evinces the necessity to review implemented procedures.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17