Proteins separation and purification by expanded bed adsorption and simulated moving bed technology

• Type: Chapter or Part of a Book
• Date: 2015

Authors

Ping Li (Author) Other; Pedro Ferreira Gomes (Author) FEUP; José Miguel Loureiro (Author) FEUP; Alírio Rodrigues (Author) FEUP

Book

Title: Continuous Processing in Pharmaceutical Manufacturing

Pages: 1-34

ISBN: 9783-527673681

Scientific classification

FOS: Engineering and technology > Chemical engineering

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

DOI: 10.1002/9783527673681.ch01

Abstract (EN): The development of techniques and methods for proteins separation and purification has been an essential prerequisite for many of the advancements made in biotechnology. Most separation and purification protocols require more than one step to achieve the desired level of protein purity. Usually, a three-step separation and purification strategy is presented, which includes capture, intermediate separation and purification, and final polishing during a downstream protein separation and purification process. This chapter describes the developments made at the Laboratory of Separation and Reaction Engineering (LSRE) for proteins separation and purification by expanded bed chromatography and salt gradient ion exchange simulated moving bed (SMB) technology. Experiments were carried out for bovine serum albumin (BSA) and myoglobin adsorption/desorption in expanded beds. Some strategies are also discussed for the selection of salt gradient and the selection of flow rate in each section of salt gradient ion exchange SMB.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 33