Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry

• Type: Article in International Scientific Journal
• Date: 2003

Authors

M. C. L. Martins (Author) Other; José Carlos Fonseca (Author) FEUP; Mário Adolfo Barbosa (Author) FEUP; B. D. Ratner (Author) Other

Journal

Title: Biomaterials

Vol. 24 No. 21

Pages: 3697-3706

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 45 Citations

Scopus - 48 Citations

Other information

Authenticus ID: P-000-F7R

DOI: 10.1016/s0142-9612(03)00244-8

Abstract (EN): Chronopotentiometry was used to study the adsorption of human serum albumin (HSA) to self-assembled monolayers with the following terminal functional groups: CH3, COOH and OH. Surfaces were characterized by X-ray photoelectron spectroscopy, water contact angle measurements and cyclic voltammetry. HSA coverage of the different SAMs was investigated by chronopotentiometry and the total amount of adsorbed protein was determined using radiolabelled albumin. Both techniques have demonstrated that HSA adsorption to the different SAM-modified electrodes increases in the following order: OH < COOH < CH3-terminated SAMs. A good correlation between coverage and total amount of HSA adsorbed was observed for long adsorption times (900 s).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10