Alzheimer's disease: Development of a sensitive label-free electrochemical immunosensor for detection of amyloid beta peptide

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Pedro Carneiro (Author) FEUP; Joana Loureiro (Author) FEUP; Cristina Delerue Matos (Author) Other; Simone Morais (Author) Other; Maria do Carmo Pereira (Author) FEUP

Journal

Title: Sensors and Actuators, B: Chemical

Vol. 239

Pages: 157-165

ISSN: 0925-4005

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 30 Citations

ISI Web of Science

Scopus - 68 Citations

INSPEC

Associated Projects

Referência: PTDC/QUI-BIQ/102827/2008 - Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00K-QG9

DOI: 10.1016/j.snb.2016.07.181

Abstract (EN): In this work, a highly sensitive label-free immunosensor for detection of the main biomarker of Alzheimer’s disease (AD), amyloid beta 1–42 (A (1–42)), is presented. A gold electrode was modified with a mercaptopropionic acid (MPA) self-assembled monolayer, electrodeposited gold nanoparticles (AuNPs) and a monoclonal antibody mAb DE2B4 to recognize A; all the relevant experimental variables were optimized. Antibodies were functionalized through chemical modification (thiolation) to promote the antibody immobilization on the AuNPs surface with proper orientation which enabled the direct detection of A(1–42). Scanning electron microscopy, square-wave voltammetry and electrochemical impedance spectroscopy were used to characterize the construction of the biosensor. Using the proposed immunosensor, A(1–42) was specifically detected within the linear range of 10–1000 pg mL−1 with a 5.2 pg mL−1 and 17.4 pg mL−1 detection and quantification limit, respectively; recovery values for the tested spiking levels ranged from 90.3 to 93.6%. The immunosensor enables rapid, accurate, precise, reproducible and highly sensitive detection (14.6%reduction mL pg−1) of A with low-cost and opens the possibilities for diagnostic ex vivo applications and research-based in vivo studies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9