Hydrogel depots for local co-delivery of osteoinductive peptides and mesenchymal stem cells

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Raquel R Maia (Author) Other; Mariana Barbosa (Author) Other; David B Gomes (Author) Other; Nuno Vale (Author) FCUP; Paula Gomes (Author) FCUP; Pedro L Granja (Author) FEUP; Cristina C Barrias (Author) Other

Journal

Title: Journal of Controlled Release

Vol. 189

Pages: 158-168

ISSN: 0168-3659

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 55 Citations

Scopus - 62 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-009-PHN

DOI: 10.1016/j.jconrel.2014.06.030

Abstract (EN): The outcome of cell-based therapies can benefit from carefully designed cell carriers. A multifunctional injectable vehicle for the co-delivery of human mesenchymal stem cells (hMSCs) and osteoinductive peptides is proposed, to specifically direct hMSCs osteogenic differentiation. The osteogenic growth peptide (OGP) inspired the design of two peptides, where the bioactive portion of OGP was flanked by a protease-sensitive linker, or its scrambled sequence, to provide faster and slower release rates, respectively. Peptides were fully characterized and chemically grafted to alginate. Both OGP analogs released bioactive fragments in vitro, at different kinetics, which stimulated hMSCs proliferation and osteogenesis. hMSCs-laden OGP-alginate hydrogels were tested at an ectopic site in a xenograft mouse model. After 4 weeks, OGP-alginate hydrogels were more degraded and colonized by vascularized connective tissue than the control (without OGP). hMSCs were able to proliferate, migrate outward the hydrogels, produce endogenous extracellular matrix and mineralize it. Moreover, OGP-groups stimulated hMSCs osteogenesis, as compared with the control. Overall, the ability of the proposed platform to direct the fate of transplanted hMSCs in loco was demonstrated, and OGP-releasing hydrogels emerged as a potentially useful system to promote bone regeneration.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11