Molecular and Cell Biology I

Code:

MD1102

Acronym:

BCM I

Keywords
Classification	Keyword
OFICIAL	Biology

Instance: 2017/2018 - 1S

Active?	Yes
Web Page:	https://moodle.up.pt/course/view.php?id=445
Responsible unit:	Mestrado Integrado
Course/CS Responsible:	Integrated Master in Dental Medicine

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIMD	84	PLANO OFICIAL 2007/2008 BOLONHA	1	-	6	90	162

Teaching language

Portuguese

Objectives

- Acquisition of theoretical and theoretical-practical skills related to the composition and the functioning of the cells
- Ability to understand the physiological characteristics of an organism as a result of different cell characteristics
- Acquisition of knowledge related to evolution and cell differentiation
- Acquisition of laboratory and group work skills

Learning outcomes and competences

At the end of this course, the student should have theoretical knowledge, ability to integrate the diverse theoretical themes and to prepare, execute and interprete obtained results from laboratory experiments.

Working method

Presencial

Program

Theoretical topics:
- Brief history of the evolution of Cell and Molecular Biology
- General characteristics of the cell
- Tools and techniques for the study of cell biology
- Prokaryotic and eukaryotic cells
- Structural and molecular organization of biological membranes
- Transmembrane transport 
- Cytoplasm: storage substances and cytoskeleton
- Organelles structure and function: Nucleus
- Nuclear transport and chromatin organization
- Organelles structure and function: endoplasmic reticulum
- Organelles structure and function: Golgi Complex
- Vesicular transport mechanisms. Lysosomes
- Organelles structure and function: Mitochondria
- Organelles structure and function: Peroxisomes
- Cell surface and extracellular matrix
- Adhesion and intercellular junctions
- Communication and cell signaling
- Cell cycle
- Cell death

Theoretical-practical and practical topics:
- Light Microscopy
- Types of preparations for microscopic observation
- Sample processing techniques 
- Different types of staining for light microscopy
- Specialized techniques of Cell Biology: cytochemistry, in situ hybridization, immunocytochemistry
- Protein separation and analysis: SDS-PAGE and Western blotting
- Application of theoretical knowledge to interprete and solve experimental questions
- Bibliographic databases
- Structure and organization of a scientific publication
 - View temporary and final preparations
-  Visualization of different subcellular structures in electron microscopy photographs

Mandatory literature

Cooper Geoffrey M.; The^cell. ISBN: 0-87893-220-7
Carlos Azevedo, Claudio Sunkel; Biologia Celular e Molecular, 5ª Edição, 2012, Lidel. ISBN: 978-972-757-692-0

Complementary Bibliography

Alberts Bruce 070; Essential Cell Biology. ISBN: 978-0-8153-4129-1
Alberts Bruce 070; Molecular biology of the cell. ISBN: 0-8153-4072-9
Lodish Harvey; Molecular cell biology. ISBN: 0-7167-4366-3

Teaching methods and learning activities

Theoretical classes of dynamic display of the syllabus, with the involvement of students in it. Theoretical-pratical classes of demonstration of the applicability of theoretical concepts, relating them to physiological and pathological conditions, as well as its importance in the context of scientific research. Integration of theoretical knowledge to interprete and solve experimental exercises. Laboratory classes for the observation of several kinds of preparations for light microscopy. Visualization and identification of subcellular structures by electron microscopy. Experimental procedures used for scientific research.

Software

Moodle

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Designation	Weight (%)
Exame	80,00
Teste	10,00
Trabalho laboratorial	10,00
Total:	100,00

Eligibility for exams

Minimum classification in the testes/exam = 7,20 (maximum = 16)
Presence in 3/4 of the classes (TP and P).
Final classification > 9.5 values

Calculation formula of final grade

The Final grade is calculated using the following values: Theory (80%) + theoretical-practical evaluation (10%) + practical evaluation (10%)

Special assessment (TE, DA, ...)

These students will be alowed to have alternative exam dates, in accordance with the law.