GLUTATHIONE AND CYSTEINE MEASUREMENT IN BIOLOGICAL SAMPLES BY HPLC WITH A GLASSY-CARBON WORKING DETECTOR

• Tipo: Artigo em Revista Científica Internacional
• Data: 1994

Autores

CARVALHO, FD (Autor) FFUP; REMIAO, F (Autor) Outra; VALE, P (Autor) Outra; TIMBRELL, JA (Autor) Outra; BASTOS, ML (Autor) FFUP; FERREIRA, MA (Autor) Outra

Revista

Título: Biomedical Chromatography

Vol. 8

Páginas: 134-136

ISSN: 0269-3879

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 48 Citações

Scopus - 47 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-001-K2V

DOI: 10.1002/bmc.1130080308

Abstract (EN): An HPLC method using a glassy carbon working detector for measuring cysteine and reduced glutathione (GSH) in biological samples was developed. Oxidized glutathione (GSSG) was also measured after reduction with glutathione reductase. This study was conducted in human plasma. Cysteine and GSH standard curves were linear in the physiological range, presenting detection limits of 22.0 pmol and 6.0 pmol respectively. Plasmatic results found were 43.92 +/- 4.15, 4.50 +/- 0.65, and 0.19 +/- 0.12 mM (means +/- SE), for cysteine, GSH and GSSG, respectively. Peak specificity for cysteine and reduced glutathione was certified by their disappearance after treatment with N-ethylmaleimide.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3