Acute and chronic effects of clofibrate and clofibric acid on the enzymes acetylcholinesterase, lactate dehydrogenase and catalase of the mosquitofish, Gambusia holbrooki

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Nunes, B (Autor) Outra; Carvalho, F (Autor) FFUP; Guilhermino, L (Autor) ICBAS

Revista

Título: Chemosphere

Vol. 57

Páginas: 1581-1589

ISSN: 0045-6535

Editora: Elsevier

Indexação

ISI Web of Knowledge - 52 Citações

Scopus - 67 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências da terra e ciências do ambiente

Outras Informações

ID Authenticus: P-000-7E0

DOI: 10.1016/j.chemosphere.2004.09.018

Abstract (EN): The objective of this study was to investigate both acute and chronic effects of clofibrate and clofibric acid on the enzymes acetylcholinesterase (AChE), lactate dehydrogenase (LDH) and catalase (CAT) of the mosquitofish (Gambusia holbrooki). AChE. commonly used as a biomarker of neurotoxicity, was determined in the total head. LDH, an important enzyme of anaerobic metabolism, was quantified in dorsal muscle, and CAT, enzyme which has been used as indicative parameter of peroxisome proliferation, was determined in the liver. Furthermore, alterations of body and liver weight were also deter-mined, through the calculation of the ratios final body weight/initial body weight, liver weight/final body weight, liver weight/gills weight and liver weight/head weight. Acute exposure of G. holbrooki to both clofibrate and clofibric acid induced a decrease in liver CAT activity, an increase in muscle LDH activity, while no effects were observed on AChE activity. However, chronic exposure did not alter significantly the enzymatic activities, suggesting reduced or null effects over these pathways, relative to effects reported in other species. No effects were observed for the calculated ratios, except a significant weight reduction for males chronically exposed to clofibrate.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9