Characterization and use of the total head soluble cholinesterases from mosquitofish (Gambusia holbrooki) for screening of anticholinesterase activity

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Nunes, B (Autor) Outra; Carvalho, F (Autor) FFUP; Guilhermino, L (Autor) ICBAS

Revista

Título: Journal of Enzyme Inhibition and Medicinal Chemistry

Vol. 20

Páginas: 369-376

ISSN: 1475-6366

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 29 Citações

Scopus - 34 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-000-256

DOI: 10.1080/14756360500094094

Abstract (EN): Inhibition of cholinesterases (ChE) has been widely used as an environmental biomarker of exposure to organophosphates (OP) and carbamate (CB) pesticides. Different ChE isoforms may be present in the same tissue and may present distinct sensitivities towards environmental contaminants. The present work characterises the soluble ChE present in mosquitofish (Gambusia holbrooki) total head homogenates, through the use of different substrates and selective inhibitors of cholinesterasic activity. Furthermore, the effects of sodium dodecylsulphate (SDS) on the enzymatic activity were investigated, both in vivo and in vitro. These results showed that acetylcholinesterase (AChE) seemed to be the predominant form present in head homogenates of G. holbrooki, despite the inhibition by tetraisopropylpyrophosphoramide (iso-OMPA) found at high concentrations. SDS was responsible for in vitro, but not in vivo, inhibitory effects. The in vitro AChE inhibitory effects of SDS was partially prevented by the use of increasing amounts of ethanol, suggesting that the inhibition was induced by an emulsion effect, which may explain the lack of effect in vivo.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8