Differential scanning calorimetry and hydrodynamic study of bacterial viruses. Effect of solution conditions

• Tipo: Artigo em Revista Científica Internacional
• Data: 2001

Autores

Mrevlishvili, GM (Autor) Outra; Sottomayor, MJ (Autor) FCUP; Ribeiro da Silva, MAV (Autor) FCUP; Mdzinarashvili, TD (Autor) Outra; Al Zaza, M (Autor) Outra; Tediashvili, M (Autor) Outra; Tushishvili, D (Autor) Outra; Chanishvili, N (Autor) Outra

Revista

Título: Journal of Thermal Analysis and Calorimetry

Vol. 66

Páginas: 103-113

ISSN: 1388-6150

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 5 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-XET

DOI: 10.1023/a:1012487615292

Abstract (EN): This paper deals with the study of the V-group, family T-even, E. Coli bacteria phage (named unphage). According to electron microscopic pictures, the geometrical parameters of this phage are 750x560 Angstrom (from head) and 900 Angstrom (from tail). Bacterial viruses genome-ds-DNA-expulsion from the phage capsid is induced by temperature and is not accompanied by heat effects (temperature interval 45-75 degreesC). Thus the temperature induced ejection of genetic material from phages is predominantly entropic. ds-DNA output from the capsid increases the viscosity of the phage suspension at least 100 times. ds-DNA output from the capsid is accompanied by a significant change of partial volume. The disruption of 1 mg of phage produces DeltaV=1.3x10(-9) m(3) which corresponds to a volume increase of 200%. This produces exothermic heat effects in closed calorimetric cells, with free volume above the measured liquid. The contraction of the tail of phage plays an important role in the injection, in the step where phage attaches the outer membrane of the host cell. The main factors of the DNA condensation and packaging in the virus head, and after its ejection through the hole with a diameter close to that of ds-DNA, are caused by the surrounding solution quality, so-called hydration forces between ds-DNA parallel packaged segments and more exactly by the difference of this parameter inside and outside the capsid of the phage.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11