Xanthone biosynthesis and accumulation in calli and suspended cells of Hypercium androsaemum

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Dias, ACP (Autor) Outra; Seabra, RM (Autor) Outra; Andrade, PB (Autor) FFUP; Ferreres, F (Autor) Outra; Fernandes Ferreira, M (Autor) Outra

Revista

Título: Plant Science

Vol. 150

Páginas: 93-101

ISSN: 0168-9452

Editora: Elsevier

Indexação

ISI Web of Knowledge - 44 Citações

Scopus - 49 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-11R

DOI: 10.1016/s0168-9452(99)00178-8

Abstract (EN): Calli and suspended cells of Hypericum androsaemum accumulated high levels of 1,3,5,6 and 1,3,6,7 oxygenated xanthones. The major compounds include simple oxygenated xanthones or derivatives with prenyl, pyran or methoxyl groups, four of them being new natural compounds. A hypothetical biosynthetic scheme is proposed based on the isolated compounds and statistical analysis. Xanthone accumulation was influenced greatly by medium factors, namely hormone supplementation. Calli grown with 4.5 mu M alpha-naphtaleneacetic acid (NAA) + 2.3 mu M kinetin (KIN) had the highest specific xanthone production (1.3% biomass dry weight (DW)), whereas suspended cells grown in similar medium accumulated a lower amount (0.87% DW). Calli displayed a negative linear relationship between total xanthone accumulation and NAA concentration, in the range of 4.5-22.5 mu M. However, in this range the xanthone 1,3,6,7/1,3,5,6 ratio and the biomass production showed a positive linear relationship with NAA concentration. Substitution of 4.5 mu M of NAA by the same molar amount of 2,4-dichlorophenoxyacetic acid (2,4-D), in the presence of 2.3 mu M of KIN, caused a decrease in xanthone accumulation in calli. The use of N(6)-benzyladenine (BA) instead of KIN reduced xanthone production, independently of the auxin used. This effect was attenuated when both hormones were present.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9