Ontogenic variations in free and esterified fatty acids during somatic embryogenesis of flax (Linum usitatissimum L.)

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Cunha, AC (Autor) Outra; Fernandes Ferreira, M (Autor) Outra

Revista

Título: Plant Science

Vol. 164

Páginas: 863-872

ISSN: 0168-9452

Editora: Elsevier

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-000-GYV

DOI: 10.1016/s0168(03)00074-8

Abstract (EN): In vitro cultures of flax (Linum usitatissimum L.) were established on MS medium and four samplings were made during the 7 weeks of culture. The samples varied from the original hypocotyl segments (HS) at t(0) and segments with incipient calli formation (HSC) after 2 weeks (t(2)), to embryogenic calli (EC), non-embryogenic calli (NEC) and somatic embryos (SE) collected after 5 (t(5)) and 7 weeks (t(7)). The respective free fatty acids (FFA) and esterified fatty acids (EFA) were extracted and analyzed, as methyl esters, by GC and GC-MS, and the data were submitted to analysis of variance (ANOVA) and PCA statistical analysis. The main FFA in all samples was 16:0, 18:0,18:1, 18:2 and 18:3. EFA fractions were more diverse containing some less common FA. The development of SE was associated with a decrease in neutral lipids. Both the total FFA and the total EFA, as fractions of total lipids, increased with the dedifferentiation and the early calli formation leveling off thereafter (t(5)-t(7)). FA variations related to dedifferentiation of hypocotyl tissues and growth of NEC, somatic embryogenesis, and development of SE were observed. Predominant or tissue-specific FA was also found. The value of 18:3/18:2 and 18:0/18:3 ratios as potential indicators of SE autotrophy and calli embryogenic capacity is discussed. (C) 2003 Published by Elsevier Science Ireland Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10