The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of alpha-3',4'-anhydrovinblastine in Catharanthus roseus (L) G. Don leaves

• Tipo: Artigo em Revista Científica Internacional
• Data: 1996

Autores

Sottomayor, M (Autor) FCUP; dePinto, MC (Autor) Outra; Salema, R (Autor) Outra; DiCosmo, F (Autor) Outra; Pedreno, MA (Autor) Outra; Barcelo, AR (Autor) Outra

Revista

Título: Plant, Cell and Environment

Vol. 19

Páginas: 761-767

ISSN: 0140-7791

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 29 Citações

Scopus - 41 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-001-E8B

DOI: 10.1111/j.1365-3040.1996.tb00412.x

Abstract (EN): Partially purified protein extracts of Catharanthus roseus leaves were able to couple catharanthine and vindoline to produce alpha-3',4'-anhydrovinblastine (AVLB) in a reaction strictly dependent on H2O2. This result, and the co-purification of peroxidase with AVLB synthetase activity, strongly suggest a peroxidase-like nature for the coupling enzyme, Only one peroxidase isoenzyme was detected in C. roseus leaves, and it was shown that this isoenzyme consists of a molecularly-heterogeneous basic peroxidase (EC 1 . 11 . 1 . 7) mainly located in the vacuole, These results suggest that a basic peroxidase located in the vacuole may be the main enzyme responsible for AVLB synthesis in C. roseus leaves, This isoenzyme was also found in cell walls where a peroxidase inhibitor was detected.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7